DnaD protein of Bacillus subtilis interacts with DnaA, the initiator protein of replication.
نویسندگان
چکیده
The yeast two-hybrid assay revealed that Bacillus subtilis DnaD, a possible component of the primosome and required for replication initiation, interacted with DnaA and DnaD itself. The mutant DnaD23 was incapable of interacting with DnaA but retained interaction with the wild-type DnaD. These results suggest that interaction between DnaD and DnaA is important for replication initiation.
منابع مشابه
The primosomal protein DnaD inhibits cooperative DNA binding by the replication initiator DnaA in Bacillus subtilis.
DnaA is an AAA+ ATPase and the conserved replication initiator in bacteria. Bacteria control the timing of replication initiation by regulating the activity of DnaA. DnaA binds to multiple sites in the origin of replication (oriC) and is required for recruitment of proteins needed to load the replicative helicase. DnaA also binds to other chromosomal regions and functions as a transcription fac...
متن کاملThe primosomal protein DnaD inhibits cooperative DNA binding by the 2 replication initiator DnaA in Bacillus subtilis 3 4 5
27 DnaA is a AAA+ ATPase and the conserved replication initiator in bacteria. Bacteria control 28 the timing of replication initiation by regulating the activity of DnaA. DnaA binds to multiple 29 sites in the origin of replication (oriC) and is required for recruitment of proteins needed to load 30 the replicative helicase. DnaA also binds to other chromosomal regions and functions as a 31 tra...
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The initiation of DNA replication requires the binding of the initiator protein, DnaA, to specific binding sites in the chromosomal origin of replication, oriC. DnaA also binds to many sites around the chromosome, outside oriC, and acts as a transcription factor at several of these. In low-G+C Gram-positive bacteria, the primosomal proteins DnaD and DnaB, in conjunction with loader ATPase DnaI,...
متن کاملOrdered association of helicase loader proteins with the Bacillus subtilis origin of replication in vivo.
The essential proteins DnaB, DnaD and DnaI of Bacillus subtilis are required for initiation, but not elongation, of DNA replication, and for replication restart at stalled forks. The interactions and functions of these proteins have largely been determined in vitro based on their roles in replication restart. During replication initiation in vivo, it is not known if these proteins, and the repl...
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The initiation of bacterial chromosome DNA replication and its regulation are critical events. DnaA is essential for initiation of DNA replication and is conserved throughout bacteria. In Escherichia coli, hydrolysis of ATP-DnaA is promoted by Hda through formation of a ternary complex with DnaA and DnaN, ensuring the timely inactivation of DnaA during the replication cycle. In Bacillus subtili...
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عنوان ژورنال:
- Journal of bacteriology
دوره 183 6 شماره
صفحات -
تاریخ انتشار 2001